Anti bovine sperm

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The objectives were to standardize some methodological and analytical aspects of a direct technique to detect sperm-bound anti-sperm antibodies ASAs in bovine semen using flow cytometry, including the effects of pre-fixation of sperm membranes with formalin buffer solution and inclusion of dead cells in the analysis. Fixation of sperm membranes had no significant effect on the percentage of IgG- or IgA-bound spermatozoa detected by flow cytometry. However, including dead cells in the analysis increased the percentage of IgG-bound spermatozoa in fixed live and dead

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George G. Ignotz, Margaret Y. Cho, Susan S.

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The Sperm Cell pp Cite as. The presence of HLA antigens on human sperm has been demonstrated by cytotoxic assay, immunofluorescence and inhibition-of-motility assay Fellous et al. Similar results were found for SLA antigen on boar sperm Vaiman

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Souza I,II ; A. Moura I,II ; A. Killian II.

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Use the link below to share a full-text version of this article with your friends and colleagues. Learn more. Volume 35Issue 1.

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Skip to search form Skip to main content. Comparative fluorescence analysis of the bovine sperm using IVA anti-CD46 antibody and lectins: probable localisation of CD46 on bovine sperm membrane. Expression of CD46 on human, mice, rat and guinea pig spermatozoa is restricted to the inner acrosomal membrane.

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Sperm are allogenic to the female genital tract; however, oviducts provide optimal conditions for survival and capacitation of these non-self cells until fertilization. Recently, we showed that oviduct-conditioned media and prostaglandin E 2 PGE 2 suppress sperm phagocytosis by polymorphonuclear neutrophils PMNs under physiological conditions. As the first step to obtain basic information, sub-confluent BOEC monolayers were co-cultured with swim-up sperm for 2 h.

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