The objectives were to standardize some methodological and analytical aspects of a direct technique to detect sperm-bound anti-sperm antibodies ASAs in bovine semen using flow cytometry, including the effects of pre-fixation of sperm membranes with formalin buffer solution and inclusion of dead cells in the analysis. Fixation of sperm membranes had no significant effect on the percentage of IgG- or IgA-bound spermatozoa detected by flow cytometry. However, including dead cells in the analysis increased the percentage of IgG-bound spermatozoa in fixed live and dead
The Sperm Cell pp Cite as. The presence of HLA antigens on human sperm has been demonstrated by cytotoxic assay, immunofluorescence and inhibition-of-motility assay Fellous et al. Similar results were found for SLA antigen on boar sperm Vaiman
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Skip to search form Skip to main content. Comparative fluorescence analysis of the bovine sperm using IVA anti-CD46 antibody and lectins: probable localisation of CD46 on bovine sperm membrane. Expression of CD46 on human, mice, rat and guinea pig spermatozoa is restricted to the inner acrosomal membrane.
Sperm are allogenic to the female genital tract; however, oviducts provide optimal conditions for survival and capacitation of these non-self cells until fertilization. Recently, we showed that oviduct-conditioned media and prostaglandin E 2 PGE 2 suppress sperm phagocytosis by polymorphonuclear neutrophils PMNs under physiological conditions. As the first step to obtain basic information, sub-confluent BOEC monolayers were co-cultured with swim-up sperm for 2 h.